Inhibition of mutagenicity by Atriplex undulate (Moq)D. Dietr. (Chenopiaceae)

RODRIGUEZ, SILVANA; SUEIRO, R.A.; ANA PAULA MURRAY; LEIRO J.M.

New York

Congreso; 2012 International Congress on Natural Products Research (ICNPR); 2012

ASP, SIF, GA, PSE and AFERP

Atriplex undulata, commonly known as Zampa crespa or Cachiyuyo, is an endemic species. It originates from Patagonia Argentine and is used as astringent and antiequimotic. Aerial parts from A. undulata, were extracted with ethanol. The ethanolic extract and its ethyl acetate partition were evaluated the direct mutagenic and antimutagenic effects. The ethyl acetate partition, which has presented a promising antimutagenic activity, was fractionated according to previous reports 1 . The experiments were carried out using the Ames test with Salmonella typhimuriumstrains TA100, TA98, TA102, TA 1535 and TA 1537. Both extract did not induce relevant increases in the number of revertant colonies in any of the tester strains assayed, at concentrations between 50 and 0.05 mg/mL. These extract also showed antimutagenic activity against sodium azide (NaN3), 9 amine acridine (9AA), 2,4,7-trinitro-9-fluorenone (TNF) and Mitomycin C. The inhibition of the mutagenic effect ranged from 47.16% to 81.29% in a concentration-dependent manner. This antimutagenic effect could be explained, at least in part, by the presence in this fraction of quercetin, which showed significantly inhibition of mutagenicity.