INQUISUR   21779
INSTITUTO DE QUIMICA DEL SUR
Unidad Ejecutora - UE
artículos
Título:
Kinetic approach for the enzymaticdetermination of levodopa and carbidopa assisted bymultivariate curveresolution-alternating least squares
Autor/es:
MARCOS GRÜNHUT; MARIANO GARRIDO; MARIA E. CENTURIÓN; BEATRIZ S. FERNÁNDEZ BAND
Revista:
ANALYTICA CHIMICA ACTA
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Año: 2010 vol. 673 p. 33 - 39
ISSN:
0003-2670
Resumen:
A combination of kinetic spectroscopic monitoring and multivariate curve resolution-alternating least
squares (MCR-ALS)wasproposed for the enzymatic determination of levodopa (LVD) and carbidopa (CBD)
in pharmaceuticals. The enzymatic reaction process was carried out in a reverse stopped-flow injection
system and monitored by UVvis spectroscopy. The spectra (292600 nm) were recorded throughout the
reaction and were analyzed by multivariate curve resolution-alternating least squares. A small calibration
matrix containing nine mixtures was used in the model construction. Additionally, to evaluate the
prediction ability of the model, a set with six validation mixtures was used. The lack of fit obtained was
4.3%, the explained variance 99.8% and the overall prediction error 5.5%. Tablets of commercial samples
were analyzed and the results were validated by pharmacopeia method (high performance liquid chromatography).
No significant differences were found (²= 0.05) between the reference values and the ones
obtained with the proposed method. It is important to note that a unique chemometric model made it
possible to determine both analytes simultaneously.
obtained with the proposed method. It is important to note that a unique chemometric model made it
possible to determine both analytes simultaneously.
²= 0.05) between the reference values and the ones
obtained with the proposed method. It is important to note that a unique chemometric model made it
possible to determine both analytes simultaneously.