Flow-Batc analyser with chemiluminiscence detection to determination of three catecholamines in pharmaceutical preparations”.

GRÜNHUT, MARCOS; MARTINS, VALDOMIRO; CENTURIÓN, MARIA EUGENIA; UGULINO DE ARAÚJO, MÁRIO; FERNÁNDEZ BAND, BEATRIZ.

ANALYTICAL LETTERS

TAYLOR & FRANCIS INC

Año: 2009

0003-2719

A novel flow-batch analyser with chemiluminescence detection was developed for determination of dopamine (DOP), norepinephrine (NOR) and epinephrine (EPI) in pharmaceutical preparations. The method is highly sensitivity for the three analytes and is based on the inhibitory effect of the mentioned catecholamines on a luminol-potassium hexacyanoferrate (III) chemiluminescence system in alkaline medium. The flow-batch parameters were optimised by univariate method and the chemical variables by using a Box-Behnken experimental design. Calibrations graphs of peak height versus DOP, NOR or EPI concentration were established by applying univariate linear regression. The limits of detection were 2.1, 1.1 and 3.3 ng mL-1 to DOP, NOR and EPI, respectively.  Pharmaceuticals preparations were analysed and the results obtained are in closed agreement with pharmacopeia method (HPLC). The sample throughput was 30 h-1.