Dual-fluorescent nanoscale coordination polymers via a mixed-ligand synthetic strategy and their use for multichannel imaging

NADOR, FABIANA; LORENZO, JULIA; NOVIO, FERNANDO; GARCÍA-PARDO, JAVIER; RUIZ-MOLINA, DANIEL; WNUK, KAROLINA; SOLORZANO, RUBÉN

CHEM NANO MAT

WILEY ONLINE LIBRARY

Año: 2017

Two rationally-designed strategies for covalent bonding of fluorescent dyes in coordination polymer nanoparticles to achieve bifunctional fluorescent nanostructures have been developed. The first strategy was based on the synthesis of the coordination polymers structured as nanoparticles by coordination of CoII ions to two different catechol ligandscontaining free functional chemical groups (dopamine and 3,4-dihydroxybenzaldehyde), and a bis-imidazol ligand (1,4-bis(imidazole-1-ylmethyl)benzene, bix). Subsequently, different dyes namely fluorescein isothiocyanate (FITC), 1-pyrenebutanoic acid hydrazide (PBH) or Alexa Fluor® 568 (A568) could be sequentially attached to the nanoparticles surface. The second strategy was focused on the pre-functionalization of catechol ligands with the corresponding dyes and afterward the coordination with the metal ions in presence of bix. In vitro studies demonstrated the internalization of the bifunctional nanoparticles and the persistence of the fluorescent properties after cell uptake without dye leaching.