INQUISUR   21779
INSTITUTO DE QUIMICA DEL SUR
Unidad Ejecutora - UE
artículos
Título:
Determination of fluoroquinolones in bovine milk samples using a MWCNT-based pipette tip solid phase extraction step prior to capillary electrophoretic separation
Autor/es:
VALERIA SPRINGER; JOHAN JACKSEN; PATRIK EK; ADRIANA G. LISTA; ASA EMMER
Revista:
JOURNAL OF SEPARATION SCIENCE
Editorial:
WILEY-V C H VERLAG GMBH
Referencias:
Lugar: Weinheim; Año: 2014 vol. 37 p. 158 - 164
ISSN:
1615-9306
Resumen:
A simple CE?UV method was developed for the simultaneous determination of ciprofloxacin, norfloxacin, and ofloxacin in milk samples. The optimum separation was obtained using a 20mMammonium dihydrogenphosphate solution with 2mMcetyltrimethylammonium bromide at pH 3.0 as the BGE. Satisfactory resolution for structurally very similar analytes, like norfloxacin and ciprofloxacin, was achieved without including any organic solvent. Milk samples were prepared using a simple/extraction procedure based on acidic protein precipitation followed by an SPE step using only 5 mg of multiwalled carbon nanotubes as the sorbent material. The LODs for the three compounds were between 7.5 and 11.6 g/L and the RSDs for the peak areas were between 2.6 and 4.9%. The complete method was applied to spiked real milk samples with satisfactory recoveries for all analytes (84?106%).