INQUISUR   21779
INSTITUTO DE QUIMICA DEL SUR
Unidad Ejecutora - UE
artículos
Título:
Nano-hydroxyapatite for use in bone tissue repair
Autor/es:
D'ELÍA NOELIA LAURA; GRAVINA ANABELA NOEL; LAIUPPA JUAN ANDRÉS; SANTILLÁN GRACIELA EDITH; MESSINA PAULA VERÓNICA
Revista:
BONE
Editorial:
ELSEVIER SCIENCE INC
Referencias:
Lugar: Amsterdam; Año: 2015 vol. 71 p. 260 - 260
ISSN:
8756-3282
Resumen:
Bone can be considered as a biological hybrid material composed of organic and inorganic components: collagen and rod-shaped hydroxyapatite (HAP) of 20?50 nm lengths. The synthetic hydroxyapatite (Ca10(PO4)6(OH)2) has been extensively used as a bone substitute material due to its chemical and structural similarities with natural mineral bone. One way to obtain HAP nanoparticles is by using self-assembled amphiphilic molecules as structure directors. This study involves different hexadecyltrimethylammonium bromide (CTAB) micellar-block copolymer organized networks. Inorganic precursors were added in sequence to the CTAB-polymer solution, followed by a hydrothermal treatment. The final product was separated from the suspension by filtration and then dried. The X-ray diffraction and infrared spectroscopy pattern of the materials synthesized corresponds to the HAP pattern. Transmission and scanning electron microscopy microphotographs show a fiber network composed by 37 nm length HAP nanorods. After treatment with simulated body fluid (SBF) a layer of HAP nanocrystals grew on the material surface; that is related to the bioactivity of the material. To confirm the samples´ biocompatibility, calvarial osteoblasts obtained from neonatal rats were exposed to the material and then, viability and cell adhesion were evaluated. A new method of HAP nanocrystals with similar shape, morphology and chemical characteristics of bone were developed. After SBF immersion, material revealed a spherulitic-like HAP layer that implies a positive physiological response and good bond ability to the host tissue. Therefore, nanomaterials obtained by the proposed synthesis could have a wide range of biomedical applications.