INQUISUR   21779
INSTITUTO DE QUIMICA DEL SUR
Unidad Ejecutora - UE
artículos
Título:
Hybrid hard - and soft - modeling of spectrophotometric data for monitoring of ciprofloxacin, oxacin and its main photodegradation products at different pH values
Autor/es:
MARIELA RAZUC, MARIANO GARRIDO, YAMILE S. CARO, CARLA M. TEGLIA, HÉCTOR C. GOICOECHEA, BEATRIZ S. FERNÁNDEZ BAND
Revista:
SPECTROCHIMICA ACTA. PART A, MOLECULAR AND BIOMOLECULAR SPECTROSCOPY.
Editorial:
PERGAMON-ELSEVIER SCIENCE LTD
Referencias:
Lugar: Amsterdam; Año: 2013 vol. 146 p. 146 - 154
ISSN:
1386-1425
Resumen:
A simple and fast online spectrophotometric method combined with a hybrid hard?soft modeling mul-tivariate curve resolution(HS MCR) was proposed for the monitoring of photodegradation reaction of ciprofloxacin under UV radiation. The studied conditions attempt to emulate the effect of sun light on these antibiotics that could be eventually present in the environment. The continuous flow system made it possible to study the ciprofloxacin degradation at different pH values almost at real time, avoiding errors that could arise from typical batch monitoring of there action. On the base of a concentration profiles obtained by previous pure soft-modeling approach, reaction pathways have been proposed for the parent compound and its photo products at different pH values. These kinetic models were use dasa constraint in the HS-MCR analysis. The kinetic profiles and the corresponding pure response profile (UV?Vis spectra) of ciprofloxacin and its main degradation products were recovered after the application of HS-MCR analysis to the spectra recorded through out the reaction. The observed behavior showed a good agreement with the photodegradation studies reported in the bibliography. Accordingly, the photodegradation reaction was studied by high performance liquid chromatography coupled with UV?Vis diode array detector (HPLC-DAD). The spectra recorded during the chromatographic analysis present a good correlation with the ones recovered by UV?Vis/HS-MCR method.