INQUISUR   21779
INSTITUTO DE QUIMICA DEL SUR
Unidad Ejecutora - UE
artículos
Título:
Cytotoxic Effects Induced by Combination of Heliantriol B2 and Dequalinium against Human Leukemic Cell Lines
Autor/es:
M. SOLEDAD VELA GUROVIC; A. MARÍA DÍAZ LANZA; MARÍA DEL CARMEN BOYANO ADÁNEZ; M. CRISTINA ESTAÑ OMAÑA; IRENE GAÑÁN GÓMEZ; A. PAULA MURRAY; PILAR SANCHO LÓPEZ
Revista:
PHYTOTHERAPY RESEARCH
Editorial:
JOHN WILEY & SONS LTD
Referencias:
Año: 2011 vol. 25 p. 603 - 610
ISSN:
0951-418X
Resumen:
Natural occurring compounds are considered an important source of antitumoral agents. In the present study, the cytotoxic potential of three pentacyclic triterpenes isolated from Chuquiraga erinacea (Asteraceae), against the human leukemic cell lines NB4 and K562 was assessed. Heliantriol B2 (HB2) showed the highest cytotoxic activity after 24 h treatment showing IC50 values of 1.98 ± 0.12 mM and 3.52 ± 0.14 mM for NB4 and K562 cells, respectively. This activity was higher than that of the reference compound dequalinium (DQA).Apoptosis and necrosis induced by HB2 in both NB4 and K562 cell lines were analysed by Annexin V/PI labeling. Mitochondrial alterations including reactive oxygen species (ROS) production and mitochondrial transmembrane potential (DYm) were also tested. The results demonstrated that HB2 induced cell death by apoptosis and necrosis and showed enhanced cytotoxic effects in combination with DQA. Besides, HB2 induced ROS overproduction in NB4 cells and a slight decrease of DYm. Consequently, our findings prompt further studies on the HB2 mechanism of action and its selectivity to tumor cells in order to assess the potential of HB2 as an agent for cancer treatment.