?? AUTOMATIC FLOW-BATCH SYSTEM FOR ON LINE SAMPLE TREATMENT AND SPECTROPHOTOMETRIC DETERMINATION OF HYDROXYPROLINE IN SAUSAGES.

BELLONI; DI NEZIO; M. PISTONESI,; CENTURION

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

AMER CHEMICAL SOC

Lugar: Washington; Año: 2012 vol. 89 p. 526 - 530

0021-8561

In this study an automatic method for sample treatment and spectrophotometric determination of hydroxyproline in commercial sausages was developed. A flow-batch system that includes the steps of sample hydrolysis and determination of the analyte was designed. The method presents a linear range between 0.60 and 3.60 g mL−1 of hydroxyproline. A relative standard deviation of 1.68% was obtained from hydroxyproline standard solution (n = 6, 1.20 g mL−1) and the detection limit was 0.12 g mL−1. The sample throughput was 1 sample h−1 while the reference method (AOAC) was carried out in about 17 h. This method employs 16 h of hydrolysis while in the proposed method the hydrolysis time was 15 min. For this purpose a pressure hydrolysis chamber with a low cost halogen lamp was used. The flow-batch system is simple and allows the use of both chambers (hydrolysis and detection) simultaneously. The obtained results with the flow-batch method are in good agreement with that obtained with the reference method. Moreover, it is good alternative to the quality control of meat products.