Determination of As(III) and As(V) in bacterial culture by Electrothermal atomic absorption spectrometry (ETAAS) After Separation With Anion Exchange Mini-column

MAIZEL, DANIELA; BALVERDI, M DEL PILAR; BALVERDI, CECILIA; FERRERO MA; SALES, ADRIANA

Buenos Aires

Congreso; 5th International Congress on Arsenic in the Environment (As2014); 2014

International Society of Groundwater for Sustainable Development (ISGSD)

A simple approach is described for the separation and determination of inorganic arsenic species using solid phase extraction and electrothermal atomic absorption spectrometry (ETAAS). A Dowex 2x10 anion exchange mini-column was used to separate As(III) and As(V). The chemical (pH, type and concentration of eluent) and physical (flow rate of sample and eluent) parameters affecting the separation were studied. Under optimized conditions, As(V) showed a strong affinity for the mini-column, while As(III) was collected in the effluent. As(V) was recovered by elution with 0.8 mol L-1 hydrochloric acid. The influence of different culture media on the separation of As(III) and As(V) was also evaluated. Inorganic species of As were detected in the free-cells supernatants from growth culture of bacteria isolates from drinking water wells of Tucumán province, with high levels contents of arsenic.