Selection of an extracellular esterase-producing microorganisms

CASTRO GR; STETTLER AO; FERRERO MA; SIÑERIZ F

JOURNAL OF INDUSTRIAL MICROBIOLOGY

Elsevier

Año: 1992 vol. 1 p. 165 - 168

0169-4146

The conventional esterase plate-screening technique has been modified in order to obtain a better differentiation of high producing strains. A short exposure to Lugol's iodine solution after colony growth enhanced the contrast between the precipitation halo and the background. Batch cultures of a selected strain characterized as belon~ng to the Bacillus subtilis group showed a high level of extracellular activity at pH 6.6 to 8.0 and 35 ~  In crude extracts the optimum enzymatic activity was obtained at 35 ~  and pH 7.0.