Biochemical properties and mechanism of action of vanadyl (IV)-aspirin complex on bone cells lines in culture, S.B.Etcheverry, P.A.M. Williams, V.C.Sálice, D.A.Barrio, E.G.Ferrer and A.M.Cortizo

ETCHEVERRY, S.B.; WILLIAMS, P. A.; SÁLICE V.C.; BARRIO, D. A.; FERRER, EG; CORTIZO A.M.

BIOMETALS

Año: 2002 vol. 15 p. 37 - 49

0966-0844

Abstract A recently synthesized vanadyl(IV) complex with aspirin [VO(aspirin)ClH2O]2, has been thoroughly investigated by physicochemical techniques. In order to support the proposed structure, stoichiometry and the coordination sphere of the vanadium center, some studies such as elemental analysis, electronic (diffuse reflectance) and vibrational (infrared) spectroscopies, magnetic susceptibility, as well as the thermal behavior, were carried out.The bioactivity of the vanadium complex (VOAspi) was evaluated on two osteoblast-like cell lines in culture, being its cytotoxic effects stronger than the vanadyl cation as assessed by morphological changes and lipid peroxidation. These effects may be partially explained through the induction of the expression of Erks (Extracellular signal-regulated kinases) and the inhibition of the PTPases (Phosphotyrosine phosphatases) present in the cellular extracts.