FARIÑA Julia Ines
Effect of chemical and metallic compounds on laccase activity of Phlebia brevispora BAFC 633
FONSECA MI; RAMOS-HRYB AB; FARIÑA JI; SAWOSTJANIK AFANASIUK SS; VILLALBA LL; ZAPATA PD
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
Lugar: Berlin; Año: 2014 vol. 30 p. 2251 - 2251
Nine aromatic compounds (sinapic acid, syringic acid, syringol, syringaldehyde, vanillic acid, vanillin, guaiacol, caffeic acid, ferulic acid) and four metallic compounds (CuSO4, AgNO3, MnSO4, and CaCl2) were tested on their ability to increase laccase (Lac) production in the ligninolytic basidiomycete Phlebia brevispora BAFC 633. Regarding fungal growth, the addition of syringaldehyde, syringol, guaiacol, sinapic acid, vanillin, ferulic acid and CuSO4 showed a positive effect; however, it decreased dramatically with the addition of AgNO3 and did not undergo changes in the presence of CaCl2 or MnSO4. Lac activity increased with the addition of all the compounds tested, depending on the concentration and the day of culture. P. brevispora BAFC 633 produced two isoenzymes, one constitutive of 60 kDa and another of 75 kDa induced by the presence of sinapic acid, MnSO4 or CuSO4. The best inducer of the enzymatic activity was CuSO4 and acts at pre-transcriptional level regulating the onset of the transcription, but it does not rule their action at the post-transcriptional and post-translational levels, for which is necessary to deepen the knowledge of all possible regulation points of gene expression.