congresos y reuniones científicas
Expresión of the inhibitory receptor CD94/NKG2A allows the identification of different subpopulations of NKT cells
SOMAGLIA, VERONICA; FASSA, SANDRA; FAINBOIM, LEONARDO; PEREZ, MARÍA EUGENIA
Congreso; First French-Argentine Immunology Congress y LVIII Reunión Anual de la Sociedad Argentina de Inmunología; 2010
Anual de la Sociedad Argentina de Inmunología
We have previously analyzed the genotype frequency of KIR genes in a Caucasian and Amerindian control population that were then compared with their frequency in two conditions where NK cells participate, like HCV infection and pregnancy. Because of their random expression, we decided to investigate cell surface expression of KIR and CD94/NKG2A in a control population of NK and NKT cells, by using a wide panel of antibodies directed against NK receptors and examined by flow citometry. We found in peripheral blood a mean frequency of NK cells of 11.05±1 of which NKbright, that seems to represent precursors of NKdim cells, represented 0.68% of total NK cells. Moreover, we identified a frequency of 5% of NKT cells. In concordance with NK cells, NKT cells also expressed, although at lower frequency, KIR genes. In contrast with NK cells, NKT cells did not show cytotoxic activity against the target cell K562. In a cohort of 28 healthy individuals, we were able to differentiate two subpopulations of NKT cells. All 28 individuals expressed the CD3low subset, characterized by a low expression of the CD94/NKG2A heterodimeric receptor (means: CD94 49.7±4.3 and NKG2A 14.1±1.9).