IAL   21557
INSTITUTO DE AGROBIOTECNOLOGIA DEL LITORAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Long noncoding RNA-mediated epigenetic reprogramming of auxin- responsive genes under far-red light
Autor/es:
FERNÁNDEZ MILMANDA G; BALLARÉ C; MAMMARELLA MF; ARIEL FD
Lugar:
Rosario
Reunión:
Congreso; GRAFOB; 2020
Resumen:
Long noncoding (lnc) RNAs have emerged as important regulators of chromatin dynamics impacting ontranscriptional programs and leading to different developmental outputs (1). It has been recently shownthat the Arabidopsis lncRNA AUXIN REGULATED PROMOTER LOOP (APOLO) can directly recognize multipleindependent loci across the Arabidopsis genome to modulate their three-dimensional chromatinconformation and activate transcription. Among a plethora of auxin-responsive genes, APOLO regulates itsneighboring gene PID in cis, as well as the homologous gene WAG2 in trans, in the context of lateral rootdevelopment (2,3). PID and WAG2 encode two kinases responsible for the sub-cellular localization of auxintransporters from the PIN family. Interestingly, it has been proposed that leaf hyponasty in response to alow red/far-red (R/FR) light ratio is mediated by PIN-dependent auxin redistribution (4,5). Here, we showthat APOLO is induced in response to low R/FR and its deregulation affects PID and WAG2 transcriptionalaccumulation in leaves. Furthermore, APOLO-RNAi knocked-down, CRISPR/Cas9-knocked-out, and35S:APOLO overexpressing plants exhibited a differential dynamics of upward leaf movement in responseto low R/FR. Collectively, our results indicate that auxin redistribution driving hyponasty in response to lightquality is mediated by a lncRNA APOLO-dependent epigenetic reprogramming of auxin-efflux regulators.