IAL   21557
INSTITUTO DE AGROBIOTECNOLOGIA DEL LITORAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Chromatin topology regulation of the HaWRKY6 locus by small RNAs derived from a non-coding RNA.
Autor/es:
ARCE, AGUSTIN L.; MANAVELLA, PABLO A.; GAGLIARDI, DELFINA; TOMASSI, ARIEL H.; CAMBIAGNO, DAMIAN A.; ARIEL, FEDERICO D.
Lugar:
Buenos Aires
Reunión:
Simposio; Simposio Fronteras en Biociencia 3; 2018
Resumen:
MicroRNAs (miRNAs) are small 21-nucleotide RNAs that post-transcriptionally regulate gene expression, controlling development and adaptation of multicellular organisms to the environment. The miRNA-mediated regulation of transcription factors is critical for the correct response to stress conditions. For instance, the miR396-mediated regulation of HaWRKY6 controls the sunflower response to temperatures. By using Arabidopsis thaliana plants expressing reporter gene GUS under the control of the HaWRKY6 promoter, we observed that this promoter works bidirectionally, transcribing HaWRKY6 in sense and a non-coding RNA (ncRNA) in anti-sense. Once transcribed, this ncRNA adopts a fold back particular structure giving rise to regulatory small RNAs. SunflowerSmall RNA Sequencing (sRNA-Seq), revealed small interfering RNAs (siRNAs)accumulation in the ncRNA and in other two regions along the locus, indicating that silencing of ncRNA depends on ncRNA-derived siRNAs. Furthermore, both accumulated siRNAs and methylation marks in the locus change chromatin conformation, triggering loop formation. Dynamics of the establishment and dissolution of these structures, leads to differential expression of HaWRKY6 and ncRNA in different tissues in sunflower.