IAL   21557
INSTITUTO DE AGROBIOTECNOLOGIA DEL LITORAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Trans-action of the APOLO long noncoding RNA
Autor/es:
ARIEL FD
Lugar:
Córdoba
Reunión:
Congreso; Congreso de Fisiología Vegetal; 2018
Institución organizadora:
SAFV
Resumen:
In eukaryotes, genome three-dimensional organization is critical for transcriptional regulation of gene expression. It is known that long noncoding RNAs (lncRNAs) can modulate chromatin conformation of spatially related genomic locations within the nucleus. Here, we show a novel mechanism by which the lncRNA APOLO (AUXIN REGULATED PROMOTER LOOP) recognizes multiple distant independent loci in the Arabidopsis thaliana genome. We found that APOLO targets are apparently not spatially associated in the nucleus, challenging the concept of a distance-based restriction for lncRNA action. Our findings indicate that APOLO recognizes its targets by sequence complementarity and the formation of DNA-RNA duplexes (R-loops). The invasion of APOLO to the target DNA decoys the plant Polycomb Repressive Complex 1 component LHP1, modulating local chromatin 3D conformation. APOLO lncRNA coordinates the expression of distal unrelated auxin-responsive genes during lateral root formation in Arabidopsis. Hence, R-loop formation and chromatin protein decoy mediate trans action of lncRNAs.