IAL   21557
INSTITUTO DE AGROBIOTECNOLOGIA DEL LITORAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
FINE TUNING OF ARGONAUTE1 STABILITY IS REGULATED BY CURLY LEAF
Autor/es:
CAMBIAGNO, DA; MANAVELLA, PA.; RÉ, DELFINA A.; TOMASSI, A; ARCE, AL
Lugar:
Paraná
Reunión:
Congreso; LIV Reunion Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2018
Institución organizadora:
SAIB
Resumen:
CURLY LEAF (CLF) is a methlytransferase that reversibly regulates gene expression by tri-methylating H3K27 of target genes. This member of the Policomb Repressor Complex 2 was recently found in a screening designed to identify miRNA-pathway co-factors. We studied the connection of CLF to the miRNA pathway and we observed that CLF regulates ARGONAUTE1 (AGO1) stability. This protein loads mature miRNAs and slices the mRNA target or stops its translation, being the main effector of miRNA-mediated silencing. Thus, we observed that clf mutant plants, with reduced levels of AGO1, have a global defect in miRNA-mediated silencing, with deregulated miRNA-targets transcript levels. Another interesting observation was that AGO1 degradation in clf mutants is even stronger when AGO1 is loaded but hampered with not cleavable targets. We found a novel connection between epigenetics and the miRNA pathway and evidence of a potential mechanism to release not cleavable targets.