IAL   21557
INSTITUTO DE AGROBIOTECNOLOGIA DEL LITORAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Three Enzymes Involved in Glucose Metabolism in Streptomyces coelicolor A3(2)
Autor/es:
M.D. ASENCIÓN DIEZ, A.M. DEMONTE, S. PEIRÚ, H. GRAMAJO, S.A. GUERRERO, A.A. IGLESIAS
Lugar:
Villa Carlos Paz, Córdoba, Argentina
Reunión:
Congreso; XLIII Reunión Anual de SAIB; 2008
Institución organizadora:
SAIB
Resumen:
EN-P02.THREE ENZYMES INVOLVED IN GLUCOSEMETABOLISM IN Streptomyces coelicolor A3(2)Asención Diez MD, Demont AM, Peirú S*, Gramajo H*, GuerreroSA, Iglesias AA.Lab. de Bqca. Microbiana y Enz. Molecular, FBCB, UNLCONICET,Sta Fe; *IBR-CONICET, Rosario. E-mail:masencion@fbcb.unl.edu.arStreptomyces are filamentous soil bacteria producing a wide range ofsecondary metabolites including more than half of the knownmicrobial antibiotics. These metabolites are synthesized indedicated biosynthetic routes linked to primary metabolism in waysnot completely understood. Looking for a deeper comprehension ofcentral metabolic pathways in Streptomyces, we characterized threeenzymes involved in glucose utilization in S. coelicolor A3(2). Thegenes glgC, glgA and gtaB, respectively coding for ADP-glucosepyrophosphorylase (EC 2.7.7.27; ADPGlcPPase), glycogensynthase (EC 2.4.1.21) and UDPGlcPPase (EC 2.7.7.9) werecloned, expressed in Escherichia coli, and the proteins purified toelectrophoretical homogeneity. The enzymes were characterized intheir kinetic properties. Allosteric effectors were found specificallyfor ADPGlcPPase. Results contribute to a better understanding ofenzymes involved in central metabolic pathways and allow tohypothesize about regulation of glucose partitioning inStreptomyces spp. Obtaining knock out mutants of these genes willfurther help to elucidate their physiological role.