IAL   21557
INSTITUTO DE AGROBIOTECNOLOGIA DEL LITORAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Effect of Subversive Substrates on Entamoeba histolytica Thioredoxin Reductase
Autor/es:
D.G. ARIAS, A.A. IGLESIAS, S.A. GUERRERO
Lugar:
Villa Carlos Paz, Córdoba, Argentina
Reunión:
Congreso; XLIII Reunión Anual de SAIB; 2008
Institución organizadora:
SAIB
Resumen:
EN-P01.EFFECT OF SUBVERSIVE SUBSTRATES ON Entamoebahistolytica THIOREDOXIN REDUCTASE ACTIVITYArias DG, Iglesias AA, Guerrero SA.Lab. de Bioquímica Microbiana y Lab. de Enzimología Molecular,FBCB, UNL-CONICET, Santa Fe. E-mail: darias@fbcb.unl.edu.arE. histolytica, usually lives and multiplies within the human gutunder reduced oxygen pressure. During tissue invasion, it is exposedto increased amounts of reactive oxygen species, which are highlytoxic for the parasite. The metabolic pathways used by this organismto cope with such environmental changes and redox homeostasis area matter of our work. Recently, we characterized in E. histolytica afunctional thioredoxin system, which is composed by threethioredoxins and a thioredoxin reductase (TRXR). The systemserves as a reduction equivalents donor for different disulfideproteins. Our data in vitro show a central role of TRXR in thefunctionality of the system, being the enzyme a potential keymolecular target for the development of new drugs to be used in thetreatment of amoebiasis. In this work, we studied the effect ofmethylene blue (MB), essential oils from Bystropogon mollis(“peperina”) and Thymus vulgaris (“tomillo”) on the TRXR activity.Results agree with the view that these compounds are subversivesubstrates of the enzyme, as they stimulate its oxidase activity with aconcomitant inhibition of the disulfide reductase activity. This workstrongly supports the potentiality of both essential oils and MB fordesigning new anti-parasite agents to treat emergent cases ofamoebiasis resistant to conventional drugs.Granted by UNL, CAI+D 2006; ANPCyT, PICTO 05 13129.