IAL   21557
INSTITUTO DE AGROBIOTECNOLOGIA DEL LITORAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
HAWRKY6, a transcription factor from Helianthus annuus, is potentially involved in plant defense pathways
Autor/es:
GIACOMELLI JI, DEZAR CA, CHAN, RL
Lugar:
Carlos Paz
Reunión:
Congreso; XLIV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB); 2008
Resumen:
WRKY transcription factors constitute a family of more than 70 members in plants and this family is divided in several subfamilies. A few members were characterized as participants of biotic and abiotic stresses responses. Here we describe a partial functional characterization of HAWRKY6, a member of group IIa of the WRKY encoding genes family. A genomic clone was isolated from a BAC library encoding a complete cDNA plus 900 bp upstream de transcription initiation site. The coding region exhibiting a WRKY domain as well as a leucine zipper was subcloned under the control of the 35S CaMV promoter in order to transform Arabidopsis plants. The promoter region presenting 3 putative W box binding sites within region comprised between -240 and -70; two sites involved in the response to jasmonate and ethylene and a c-AMP response element, was cloned directing the GUS reporter gene expression in order to transform plants and establish the expression pattern. .In parallel, expression studies performed with qRT-PCR indicated that this gene is strongly induced by SA and ACC as well as by the infection caused by Psudomonas syringae and repressed by MeJA. Moreover, ACC treatment reverted the MeJA repression effect. We postulate that HAWRKY6 may participate somehow in the SA/JA/ET pathways in response to pathogen infections. Experimental work is in progress in order to corroborate this hypothesis.