CONICET | Buscador de Institutos y Recursos Humanos

&amp;amp;amp;amp;lt;!-- /* Font Definitions */ @font-face {font-family:TimesH-Roman; panose-1:0 0 0 0 0 0 0 0 0 0; mso-font-charset:0; mso-generic-font-family:auto; mso-font-format:other; mso-font-pitch:auto; mso-font-signature:3 0 0 0 1 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman"; mso-ansi-language:ES-TRAD; mso-fareast-language:ES-TRAD;} @page Section1 {size:612.0pt 792.0pt; margin:70.85pt 3.0cm 70.85pt 3.0cm; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --&amp;amp;amp;amp;gt; &amp;amp;amp;lt;!-- /* Font Definitions */ @font-face {font-family:SimSun; panose-1:2 1 6 0 3 1 1 1 1 1; mso-font-alt:&amp;amp;amp;#23435;&amp;amp;amp;#20307;; mso-font-charset:134; mso-generic-font-family:auto; mso-font-pitch:variable; mso-font-signature:3 135135232 16 0 262145 0;} @font-face {font-family:"@SimSun"; panose-1:2 1 6 0 3 1 1 1 1 1; mso-font-charset:134; mso-generic-font-family:auto; mso-font-pitch:variable; mso-font-signature:3 135135232 16 0 262145 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:SimSun; mso-ansi-language:EN-GB;} @page Section1 {size:595.3pt 841.9pt; margin:70.85pt 3.0cm 70.85pt 3.0cm; mso-header-margin:35.4pt; mso-footer-margin:35.4pt; mso-paper-source:0;} div.Section1 {page:Section1;} --&amp;amp;amp;gt; &lt;!-- /* Font Definitions */ @font-face {font-family:SimSun; panose-1:2 1 6 0 3 1 1 1 1 1; mso-font-alt:&#23435;&#20307;; mso-font-charset:134; mso-generic-font-family:auto; mso-font-pitch:variable; mso-font-signature:3 135135232 16 0 262145 0;} @font-face {font-family:"@SimSun"; panose-1:2 1 6 0 3 1 1 1 1 1; mso-font-charset:134; mso-generic-font-family:auto; mso-font-pitch:variable; mso-font-signature:3 135135232 16 0 262145 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:SimSun; mso-ansi-language:EN-GB;} @page Section1 {size:595.3pt 841.9pt; margin:70.85pt 3.0cm 70.85pt 3.0cm; mso-header-margin:35.4pt; mso-footer-margin:35.4pt; mso-paper-source:0;} div.Section1 {page:Section1;} --&gt;  Reactive oxygen species (ROS) are toxic molecules produced as a consequence of aerobic respiration. Mitochondria are the major source of ROS within the cell, and as a result, numerous proteins have evolved to prevent or repair oxidative damage in this organelle. The human OXR1 (oxidation resistance) gene is a member of a conserved family of eukaryotic genes and is involved in the prevention of oxidative DNA damage. Homologues are present in many eukaryotic organisms from yeast to humans. OXR1 of yeast and humans are oxidative and heat stress inducible genes whose products localize to the mitochondria. The most highly conserved region of OXR proteins is the carboxyl-terminal half, which contains a TLDc domain and a calcium binding EF-hand motif. We identified two genes in Arabidopsis, named AtOXR1 (At2g05590) and AtOXR2 (At4g39870), with homology to human the OXR1 gene. To gain insight into the mechanisms of AtOXR gene regulation, we have analyzed the upstream regions for both genes and observed GUS reporter expression mainly in vascular tissues of seedling roots. Moreover, available data show high expression levels in dry seeds and in mature pollen for both genes. Since hOXR1 can complement the peroxide sensitivity of the yeast OXR1 mutant when localized in yeast mitochondria, we made GFP and HA-tagged fusions to identify the cellular localization and constructions to complement the yeast OXR1 mutant with both Arabidopsis genes. Furthermore, in order to determine if the Arabidopsis genes are functionally related with their homologues, we made use of the spontaneous mutator phenotype of E. coli oxidative repair-deficient mutants to study their ability to protect cells from oxidative DNA damage.