IAL   21557
INSTITUTO DE AGROBIOTECNOLOGIA DEL LITORAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
A gene implicated in heme a synthesis for COX assembly, is involved in plant embryogenesis and senescence processes.
Autor/es:
MANSILLA, NATANAEL; GARCÍA LUCILA; GONZALEZ, DANIEL H.; WELCHEN, ELINA
Reunión:
Congreso; XLVII Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular (SAIB).; 2012
Resumen:
Cytochrome c oxidase (CcO) biogenesis requires more than 20 accessory proteins implicated in copper and heme a insertion, among other processes. COX10, a farnesyltransferase that catalyzes the conversion of heme b to heme o, is the limiting factor in heme a biosynthesis and would be essential for its insertion in the COX1 subunit in yeast. By complementation analysis of a yeast cox10 mutant, we obtained information about the functionality of the putative Arabidopsis COX10 protein. We observed that AtCOX10 is able to restore growth on non-fermentable carbon sources when expressed in yeast cox10 null mutant. We also have studied Arabidopsis mutant plants with a T-DNA insertion in the coding region of the AtCOX10 gene. Segregation analysis of heterozygote mutants demonstrated that was not possible to obtain AtCOX10 knockout plants. Siliques of these plants contained 25% abnormal seeds with embryos arrested at early stages of development. According to this, AtCOX10 promoter-GUS reporter expression was observed in embryos at the same age, and the phenotype was restored in Arabidopsis homozygous mutants plants complemented with the AtCOX10 expressed under the own promoter or by the constitutive 35S-CaMV promoter. These results indicate that AtCOX10 function is essential during embryogenesis. In addition, heterozygous mutants reached senescence before wild-type plants, suggesting a function of COX10 protein during both stages of plant development.