The AtIPB gene induces response mechanisms related with pathogen attack in Arabidopsis plants.

COLOMBATTTI, FRANCISCO; ANDRADE, ANDREA; GARCÍA LUCILA; ALEMANO, SERGIO; GONZALEZ, DANIEL H.; WELCHEN, ELINA.

Mendoza

Congreso; XLVII Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular (SAIB).; 2012

In this work, we analyze the effect of AtIPB (Increased Plant Biomass), a gene that encodes a nuclear protein with unknown functions in plants. To determine the role of AtIBP, we generated overexpressing (OE) plants and obtained an insertional mutant in the coding region of this gene. Under normal growth conditions, OE plants exhibited increased biomass (hence the gene name), higher ROS levels and increased content of scavengers like ascorbic acid and glutathione. At the transcriptional level, higher levels of Pathogen Related (PR) transcripts (PR1 and PR5) were observed. We also evaluated the connection between hormones and biotic stress responses in our plants. Through mass spectrometry, we determined that OE plants contained higher salicylic acid (SA) levels than wild-type plants. Moreover, OE and mutant seedlings showed different transcriptional dose-response curves when grown on MS medium containing 0.5 mM SA. In order to evaluate the effect of AtIPB in plant responses to biotic stress, we are performing survival assays of different pathogens on these plants. The results suggest that AtIPB would be a candidate to obtain plants better prepared to overcome biotic stress growing conditions.