Methods to study transcription factor structure and function

VIOLA IL; (EDITOR GONZÁLEZ DH)

Plant Transcription Factors. Evolutionary, Structural and Functional Aspects

Elsevier/Academic Press

Año: 2015; p. 13 - 33

The study of transcription factors comprises the analysis of their roles in the regulation of gene expression and how these changes in gene expression affect cell/organism functions. A complete view of transcription factor action at the molecular level requires the knowledge of their target genes and binding partners, of the effect of these interactions on gene expression, and of the mechanisms involved in the recognition of specific DNA sequences, among others. In this chapter, we summarize the different methods usually employed to study transcription factor action both in vivo and in vitro and discuss how the integration of different approaches can help to elucidate the molecular mechanisms involved in this action.