IAL   21557
INSTITUTO DE AGROBIOTECNOLOGIA DEL LITORAL
Unidad Ejecutora - UE
artículos
Título:
Light triggers the miRNA-biogenetic inconsistency for de-etiolated seedling survivability in Arabidopsis thaliana
Autor/es:
VICZIÁN, ANDRÁS; ARCE, AGUSTIN L.; DOLDE, ULLA; NAGY, FERENC; CHOI, SUK WON; JUNG, HYUN JU; ACHKAR, NATALIA P.; WENKEL, STEPHAN; CHO, SEOK KEUN; RYU, MOON YOUNG; KIM, GU MIN; MANAVELLA, PABLO; MOLNÁR, ATTILA; YANG, SEONG WOOK
Revista:
MOLECULAR PLANT
Editorial:
OXFORD UNIV PRESS
Referencias:
Lugar: Oxford; Año: 2020 vol. 13 p. 431 - 445
ISSN:
1674-2052
Resumen:
The shift of dark-grown seedlings into light causes enormous transcriptome changes followed by a dramatic developmental transition. Here, we show that miRNA biogenesis also undergoes regulatory changes during de-etiolation. Etiolated seedlings maintain low levels of primary-miRNAs (pri-miRNAs) and miRNA processing core proteins, such as Dicer-like 1 (DCL1), SERRATE (SE) and HYPONASTIC LEAVES 1 (HYL1), whereas during de-etiolation, both pri-miRNAs and the processing components accumulated to high levels. However, most miRNA levels did not notably increase in response to light. To reconcile this inconsistency, we demonstrate that an unknown suppressor decreases miRNA-processing activity and light-induced SMALL RNA DEGRADING NUCLEASE 1 (SDN1) shortens the half-life of several miRNAs in de-etiolated seedlings. Taken together, we suggest a novel mechanism, miRNA-biogenetic inconsistency, which accounts for the intricacy of miRNA biogenesis during de-etiolation. This mechanism is essential for the survival of de-etiolated seedlings after long-term skotomorphogenesis and their optimal adaptation to ever-changing light conditions.