Molecular characterization and interactome analysis of Trypanosoma cruzi Tryparedoxin II

ARIAS DG; PIÑEYRO MD; IGLESIAS AA; GUERRERO SA; ROBELLO C

JOURNAL OF PROTEOMICS

ELSEVIER SCIENCE BV

Lugar: Amsterdam ; Año: 2015 vol. 120 p. 95 - 104

1874-3919

Trypanosoma cruzi, the causative agent of Chagas disease, possesses two tryparedoxins(TcTXNI and TcTXNII), belonging to the thioredoxin superfamily. TXNs are oxidoreductases whichmediate electron transfer between trypanothione and peroxiredoxins. This constitutes a differencewith the host cells, in which these activities are mediated by thioredoxins, and this difference makesTXNs an attractive target for drug development. In a previous work we characterized TcTXNI,including the redox interactome. In this work we extend the study to TcTXNII. We demonstrate thatTcTXNII is a transmembrane protein anchored to the surface of the mitochondria and endoplasmicreticulum, with a cytoplasmatic orientation of the redox domain, and expressed during themetacyclogenesis process. In order to continue with the characterization of the redox interactome of T.cruzi, we designed an active site mutant TcTXNII lacking the resolving cysteine, and through theexpression of this mutant protein and incubation with T. cruzi proteins, heterodisulfide complexeswere isolated by affinity chromatography and identified by mass spectrometry. This allowed us toidentify sixteen TcTXNII interacting proteins, which are involved in a wide range of cellular processes,indicating the relevance of TcTXNII, and contributing to our understanding of the redox interactome ofT. cruzi.Ms. Ref. No.: JPROT-D-14-00744R1 Title: Molecular characterization and interactome analysis of Trypanosoma cruzi Tryparedoxin II Journal of Proteomics Dear Dr. Carlos Robello, I am pleased to inform you that your paper "Molecular characterization and interactome analysis of Trypanosoma cruzi Tryparedoxin II" has been accepted for publication in % JOURNALFULLTITLE%. When your paper is published on ScienceDirect, you want to make sure it gets the attention it deserves. To help you get your message across, Elsevier has developed a new, free service called AudioSlides: brief, webcast-style presentations that are shown (publicly available) next to your published article. This format gives you the opportunity to explain your research in your own words and attract interest. You will receive an invitation email to create an AudioSlides presentation shortly. For more information and examples, please visit http://www.elsevier.com/audioslides. Thank you for submitting your work to Journal of Proteomics. Yours sincerely, Juan J. Calvete, PhD Editor-in-Chief Journal of Proteomics