IAL 21557
INSTITUTO DE AGROBIOTECNOLOGIA DEL LITORAL
Unidad Ejecutora - UE
artículos
Título:
REDOX METABOLISM IN TRYPANOSOMA CRUZI: FUNCTIONAL CHARACTERIZATION OF TRYPAREDOXINS REVISITED
Autor/es:
DIEGO G. ARIAS; VANINA E. MÁRQUEZ; MARIA L. CHIRIBAO; FERNANDA GADHELA; CARLOS ROBELLO; ALBERTO A. IGLESIAS; SERGIO A. GUERRERO
Revista:
FREE RADICAL BIOLOGY AND MEDICINE
Editorial:
ELSEVIER SCIENCE INC
Referencias:
Año: 2013 vol. 63 p. 65 - 77
ISSN:
0891-5849
Resumen:
Tryparedoxins (TXNs) are multipurpose oxidoreductases from trypanosomatids that transfer reducing equivalents from trypanothione to different thiol proteins. In Trypanosoma cruzi, two genes coding for TXN like proteins have been identified: TXNI, previously characterized as a cytoplasmic protein, and TXNII, a putative tail-anchored membrane protein. In this work, we performed a comparative functional characterization of T. cruzi TXNs. Particularly, we cloned the gene region coding for the soluble version of TXNII for its heterologous expression. The truncated recombinant protein (without its 22 C-terminal transmenbrane amino acids) showed TXN activity. It was also able to transfer reducing equivalents from trypanothione, glutathione or dihydrolipoamide to different acceptors, including methionine sulfoxide reductases and peroxiredoxins. Results support the occurrence and functionality of a second tryparedoxin, which appears as a new component in redox scenario in T. cruzi.
