INVESTIGADORES
CENTRON Daniela
congresos y reuniones científicas
Título:
Evolución de los Integrones de clase 2
Autor/es:
DANIELA CENTRON
Lugar:
Bahía, Brasil
Reunión:
Conferencia; 9- III Seminario de Resistencia Bacteriana, II Seminario de Resistencia Microbiana; 2008
Institución organizadora:
Univerdiad da Bahia
Resumen:
Introduction: Ab is considered a serious threat in the hospital environment over the world being some strains resistant to almost all available antibacterial agents. Integrons are genetic elements that contain the components of a site-specific recombination system that recognizes and captures mobile gene cassettes. The type 2 integron integrase is not functional, this might be the reason for the few new rearrangements found There is hardly few evidence of the distribution of this class of integron in multiresistant Ab isolates. Objectives: The aim of this study was to evaluate the dispersion and characterization of class 2 integrons among 213 Ab multiresistant clinical isolates from 14 hospital from Buenos Aires and 4 distinct regions of Chile. Methods: Total DNA was extracted, subjected to PCR amplifications for the intI2 gene and PCR cartography in order of characterize the integrons. Results: We found a high prevalence of class 2 integrons in Ab (53%). Among the class 2 integron positive isolates, we found 9 different genetic structures. Unexpectedly, the novel rearrangement In2-8 previously found in three Argentinean Ab isolates, was also detected in 6 Chilean samples. In addition, a great diversity of class 2 integron in our Ab isolates was found, which is in contrast to the available literature for this class of integrons. Conclusion: Our study shows a huge distribution of this element among Ab clinical isolates from Argentina and Chile, revealing a particular geographical behavior. We thought that the mechanisms of construction of new rearrangements in some species are not defined yet.