Genetic characterization of Vibrio cholerae isolates from Argentina by V. cholerae repeated sequences–polymerase chain reaction

NANCY CLAUDIA CASTANEDA, MARIANA PICHEL, BETINA ORMAN, NORMA BINSZTEIN, PAUL H. ROY, DANIELA CENTRON

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASES

Elsevier

Lugar: Amsterdam; Año: 2005 vol. 53 p. 175 - 183

0732-8893

We have developed a novel typing method based on Vibrio cholerae repeat sequences (VCR) using primers directed out of the VCR sequences. To evaluate the VCR–polymerase chain reaction (PCR) as a typing system, 2 categories, efficacy and efficiency, were analyzed in 69 strains of human and environmental V. cholerae O1 toxigenic and nontoxigenic, and non-O1 strains isolated since 1992–2000 from Argentina. The discriminatory power (0.91), stability (0.95), reproducibility (1), typeability (1), rapidity, accessibility, as well ease of use, indicated that the VCR-PCR method provides an alternative useful tool for molecular epidemiology of V. cholerae. The VCR-PCR ofVibrio cholerae repeat sequences (VCR) using primers directed out of the VCR sequences. To evaluate the VCR–polymerase chain reaction (PCR) as a typing system, 2 categories, efficacy and efficiency, were analyzed in 69 strains of human and environmental V. cholerae O1 toxigenic and nontoxigenic, and non-O1 strains isolated since 1992–2000 from Argentina. The discriminatory power (0.91), stability (0.95), reproducibility (1), typeability (1), rapidity, accessibility, as well ease of use, indicated that the VCR-PCR method provides an alternative useful tool for molecular epidemiology of V. cholerae. The VCR-PCR ofV. cholerae O1 toxigenic and nontoxigenic, and non-O1 strains isolated since 1992–2000 from Argentina. The discriminatory power (0.91), stability (0.95), reproducibility (1), typeability (1), rapidity, accessibility, as well ease of use, indicated that the VCR-PCR method provides an alternative useful tool for molecular epidemiology of V. cholerae. The VCR-PCR ofV. cholerae. The VCR-PCR of V. cholerae isolates showed 29 patterns, of which pattern 1 represented 68% of the V. cholerae O1 isolates, supporting the hypothesis that a clone with epidemic behavior was responsible for the epidemic in Latin America. These results showed a good correlation and a better epidemiologic analysis when the results were compared in parallel with repetitive extragenic palindromic sequences–PCR. In conclusion, VCR-PCR showed excellent performance as a typing method for cholera surveillance programs.isolates showed 29 patterns, of which pattern 1 represented 68% of the V. cholerae O1 isolates, supporting the hypothesis that a clone with epidemic behavior was responsible for the epidemic in Latin America. These results showed a good correlation and a better epidemiologic analysis when the results were compared in parallel with repetitive extragenic palindromic sequences–PCR. In conclusion, VCR-PCR showed excellent performance as a typing method for cholera surveillance programs.