INVESTIGADORES
CENTRON Daniela
artículos
Título:
Achromobacter xylosoxidans: An Emerging Pathogen Carrying Different Elements Involved in Horizontal Genetic Transfer
Autor/es:
TRAGLIA GM, ALMUZARA M, MERKIER AK, ADAMS C, GALANTERNIK L, VAY C, CENTRÓN C, RAMÍREZ MS
Revista:
CURRENT MICROBIOLOGY
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2012 vol. 65 p. 673 - 678
ISSN:
0343-8651
Resumen:
In the last few years, numerous cases of multidrug-
resistant Achromobacter xylosoxidans infections have
been documented in immunocompromised and cystic
fibrosis patients. To gain insights into the molecular mechanisms
and mobile elements related to multidrug resistance
in this bacterium, we studied 24 non-epidemiological
A. xylosoxidans clinical isolates from Argentina. Specific
primers for plasmids, transposons, insertion sequences,
blaampC, intI1, and intI2 genes were used in PCR reactions.
The obtained results showed the presence of wide host range
IncP plasmids in ten isolates and a high dispersion of class 1
integrons (n = 10) and class 2 integrons (n = 3). Four arrays
in the variable region (vr) of class 1 integrons were identified
carrying different gene cassettes as the aminoglycoside
resistance aac(60)-Ib and aadA1, the trimethoprim resistance
dfrA1 and dfrA16, and the b-lactamase blaOXA-2. In only one
of the class 2 integrons, a vr was amplified that includes
sat2-aadA1. The blaampC gene was found in all isolates,
confirming its ubiquitous nature. Our results show that
A. xylosoxidans clinical isolates contain a rich variety of
genetic elements commonly associated with resistance genes
and their dissemination. This supports the hypothesis that
A. xylosoxidans is becoming a reservoir of horizontal genetic
transfer elements commonly involved in spreading antibiotic
resistance.