Cobalt chloride protects the heart after a global ischemic insult

GUTIERREZ, CHRISTOPHER P. A.; FARCY, NICOLE; BONAZZOLA, PATRICIA; CASTILLA, ROCÍO

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2020

Sociedad Argentina de Investigación Clínica

Introduction: Ischemia-reperfusion (I/R) is one of the main cardiovascular risk factors and leads to heart contractile and energy dysfunction. I/R-induced damage is reduced by ischemic postconditioning. CoCl₂ has properties to function as a postconditioning agent, since it can trigger transcriptional changes that resemble the response to a hypoxic event under normoxic conditions.Objectives: To evaluate CoCl₂ as a postconditioning therapeutic tool after a myocardial and arterial I/R.Materials and Methods: Isolated adult Wistar rats hearts were arterially perfused at 37ºC by Langendorff method, paced at 3 Hz, exposed to 30 min ischemia followed by 45 min reperfusion (R) in the presence or absence of 0.23 mM CoCl2 which was maintained or removed after 20 min of R.Aortic contractility was evaluated in an isolated organ bath trough incubation with cumulative noradrenaline (NA) doses. After NA washing, 20 min of simulated arterial ischemia (SI) and R with or without CoCl₂ was performed, and NA response was re-evaluated.Results: During R, the presence of CoCl₂ did not alter the cardiac resting pressure, nor the perfusion pressure, but increased the developed pressure (p