INVESTIGADORES
CASTILLA LOZANO Maria Del Rocio
congresos y reuniones científicas
Título:
Phisyological and biochemical alterations induced by hexachlorobenzene are prevented by an angiotensin II receptor type 1 antagonist
Autor/es:
ROMERO CAÍMI, GISELLE; BONAZZOLA, PATRICIA; GORZALCZANY, SUSANA; ROSÓN, MARÍA INÉS; VILLA ETCHEGOYEN, CECILIA; DEHEZA, SAHIRA; ALVAREZ, LAURA; CASTILLA, ROCÍO
Lugar:
Buenos Aires
Reunión:
Congreso; IV INTERNATIONAL CONGRESS IN TRANSLATIONAL MEDICINE; 2018
Institución organizadora:
Maestría Internacional en Ciencias Biomédicas (IMBS)
Resumen:
In previous work we showed that environmental dioxine-type hexachlorobenzene (HCB) increases blood pressure (BP) in rats, causing alterations in arterial structure and function. Thyroid hormone, angiotensin II receptor type 1 (AT1) and endothelial nitric oxide synthase (eNOS) are involved in this toxic effect. Here we study whether AT1 receptor antagonist Losartan (L) can prevent alterations caused by HCB intoxication.Male Wistar rats were treated with HCB (500 mg/kg/bw) with or without L (30 mg/kg/day) for 45 days. BP was measured throughout treatment. After 45 days, we analyzed: 1) heart contractile and energetic response by simultaneous mechanical and heat assays in hearts arterially perfused at 37 ºC by Langendorff method, paced at 3 Hz, and exposed to 25 min ischemia followed by 45 min reperfusion (R); 2) kidney function by uremia determination; 3) aortic morphology through thickness and cell number; 4) vascular physiology as arterial contractility in aortic rings contracted with phenylephrine (P), and relaxed with acetylcholine (Ach) and nitroprusside (N) (isometric tension); and 5) molecular markers potentially involved in the mechanism of toxic action by western blot.HCB-treated rats showed an increase in BP (145.5±5.1 mmHg), which was prevented by simultaneous administration of L (110.5±8.2 mmHg, p