High genetic diversity among viruses related to sugarcane mosaic disease in Tucumán, Argentina.

M. F. PERERA, M. P.FILIPPONE, M. I. CUENYA, J. RAMALLO, M. L. GARCÍA AND A. P. CASTAGNARO

Caims, Australia

Conferencia; Tropical Crop Biothecnology Conference; 2006

Tropical Crop Biothecnology Conference

Sugarcane mosaic is the most widespread viral disease affecting sugarcane production and causing significant yield losses. Historically, the causal agent of the disease was attributed to a potyvirus, Sugarcane mosaic virus (SCMV) with numerous strains, butSugarcane mosaic virus (SCMV) with numerous strains, but Sorghum mosaic virus (SrMV) is also known to infect sugarcane under natural conditions and both of them are considered as the causal agents of sugarcane mosaic disease (Grisham, 2000). In Argentina, the first report of these viruses was by Bennet (1941), who determined the presence of the SCMV strain B by the characterization of symptoms produced on differential hosts and later Ramallo (1981), detected the presence of the strains A and F using a similar approach. Recently the predominance of strain E was determined by Fontana et al. (2004) analyzing a region of the coat protein (CP) gene by RT-PCR and RFLP. Our objective is to study the genetic diversity of the viruses associated with sugarcane mosaic disease in Tucumán (Argentina), analyzing the sequence variability within the CP-coding region. For this purpose, leaf samples showing severe and common mosaic symptoms, were collected from 61 sugarcane plants (corresponding to 37 genotypes of sugarcane) in 6 locations throughout the Tucumán sugarcane crop area. The RT-PCR was performed as Yang and Mirkov, (1997), using the primers SCMV R3-F3, and SCMV R3-F4 designed by Alegria et al. (CP) gene by RT-PCR and RFLP. Our objective is to study the genetic diversity of the viruses associated with sugarcane mosaic disease in Tucumán (Argentina), analyzing the sequence variability within the CP-coding region. For this purpose, leaf samples showing severe and common mosaic symptoms, were collected from 61 sugarcane plants (corresponding to 37 genotypes of sugarcane) in 6 locations throughout the Tucumán sugarcane crop area. The RT-PCR was performed as Yang and Mirkov, (1997), using the primers SCMV R3-F3, and SCMV R3-F4 designed by Alegria et al. (CP) gene by RT-PCR and RFLP. Our objective is to study the genetic diversity of the viruses associated with sugarcane mosaic disease in Tucumán (Argentina), analyzing the sequence variability within the CP-coding region. For this purpose, leaf samples showing severe and common mosaic symptoms, were collected from 61 sugarcane plants (corresponding to 37 genotypes of sugarcane) in 6 locations throughout the Tucumán sugarcane crop area. The RT-PCR was performed as Yang and Mirkov, (1997), using the primers SCMV R3-F3, and SCMV R3-F4 designed by Alegria et al. conditions and both of them are considered as the causal agents of sugarcane mosaic disease (Grisham, 2000). In Argentina, the first report of these viruses was by Bennet (1941), who determined the presence of the SCMV strain B by the characterization of symptoms produced on differential hosts and later Ramallo (1981), detected the presence of the strains A and F using a similar approach. Recently the predominance of strain E was determined by Fontana et al. (2004) analyzing a region of the coat protein (CP) gene by RT-PCR and RFLP. Our objective is to study the genetic diversity of the viruses associated with sugarcane mosaic disease in Tucumán (Argentina), analyzing the sequence variability within the CP-coding region. For this purpose, leaf samples showing severe and common mosaic symptoms, were collected from 61 sugarcane plants (corresponding to 37 genotypes of sugarcane) in 6 locations throughout the Tucumán sugarcane crop area. The RT-PCR was performed as Yang and Mirkov, (1997), using the primers SCMV R3-F3, and SCMV R3-F4 designed by Alegria et al. (CP) gene by RT-PCR and RFLP. Our objective is to study the genetic diversity of the viruses associated with sugarcane mosaic disease in Tucumán (Argentina), analyzing the sequence variability within the CP-coding region. For this purpose, leaf samples showing severe and common mosaic symptoms, were collected from 61 sugarcane plants (corresponding to 37 genotypes of sugarcane) in 6 locations throughout the Tucumán sugarcane crop area. The RT-PCR was performed as Yang and Mirkov, (1997), using the primers SCMV R3-F3, and SCMV R3-F4 designed by Alegria et al. (CP) gene by RT-PCR and RFLP. Our objective is to study the genetic diversity of the viruses associated with sugarcane mosaic disease in Tucumán (Argentina), analyzing the sequence variability within the CP-coding region. For this purpose, leaf samples showing severe and common mosaic symptoms, were collected from 61 sugarcane plants (corresponding to 37 genotypes of sugarcane) in 6 locations throughout the Tucumán sugarcane crop area. The RT-PCR was performed as Yang and Mirkov, (1997), using the primers SCMV R3-F3, and SCMV R3-F4 designed by Alegria et al. conditions and both of them are considered as the causal agents of sugarcane mosaic disease (Grisham, 2000). In Argentina, the first report of these viruses was by Bennet (1941), who determined the presence of the SCMV strain B by the characterization of symptoms produced on differential hosts and later Ramallo (1981), detected the presence of the strains A and F using a similar approach. Recently the predominance of strain E was determined by Fontana et al. (2004) analyzing a region of the coat protein (CP) gene by RT-PCR and RFLP. Our objective is to study the genetic diversity of the viruses associated with sugarcane mosaic disease in Tucumán (Argentina), analyzing the sequence variability within the CP-coding region. For this purpose, leaf samples showing severe and common mosaic symptoms, were collected from 61 sugarcane plants (corresponding to 37 genotypes of sugarcane) in 6 locations throughout the Tucumán sugarcane crop area. The RT-PCR was performed as Yang and Mirkov, (1997), using the primers SCMV R3-F3, and SCMV R3-F4 designed by Alegria et al. (CP) gene by RT-PCR and RFLP. Our objective is to study the genetic diversity of the viruses associated with sugarcane mosaic disease in Tucumán (Argentina), analyzing the sequence variability within the CP-coding region. For this purpose, leaf samples showing severe and common mosaic symptoms, were collected from 61 sugarcane plants (corresponding to 37 genotypes of sugarcane) in 6 locations throughout the Tucumán sugarcane crop area. The RT-PCR was performed as Yang and Mirkov, (1997), using the primers SCMV R3-F3, and SCMV R3-F4 designed by Alegria et al. (CP) gene by RT-PCR and RFLP. Our objective is to study the genetic diversity of the viruses associated with sugarcane mosaic disease in Tucumán (Argentina), analyzing the sequence variability within the CP-coding region. For this purpose, leaf samples showing severe and common mosaic symptoms, were collected from 61 sugarcane plants (corresponding to 37 genotypes of sugarcane) in 6 locations throughout the Tucumán sugarcane crop area. The RT-PCR was performed as Yang and Mirkov, (1997), using the primers SCMV R3-F3, and SCMV R3-F4 designed by Alegria et al. (SrMV) is also known to infect sugarcane under natural conditions and both of them are considered as the causal agents of sugarcane mosaic disease (Grisham, 2000). In Argentina, the first report of these viruses was by Bennet (1941), who determined the presence of the SCMV strain B by the characterization of symptoms produced on differential hosts and later Ramallo (1981), detected the presence of the strains A and F using a similar approach. Recently the predominance of strain E was determined by Fontana et al. (2004) analyzing a region of the coat protein (CP) gene by RT-PCR and RFLP. Our objective is to study the genetic diversity of the viruses associated with sugarcane mosaic disease in Tucumán (Argentina), analyzing the sequence variability within the CP-coding region. For this purpose, leaf samples showing severe and common mosaic symptoms, were collected from 61 sugarcane plants (corresponding to 37 genotypes of sugarcane) in 6 locations throughout the Tucumán sugarcane crop area. The RT-PCR was performed as Yang and Mirkov, (1997), using the primers SCMV R3-F3, and SCMV R3-F4 designed by Alegria et al. (CP) gene by RT-PCR and RFLP. Our objective is to study the genetic diversity of the viruses associated with sugarcane mosaic disease in Tucumán (Argentina), analyzing the sequence variability within the CP-coding region. For this purpose, leaf samples showing severe and common mosaic symptoms, were collected from 61 sugarcane plants (corresponding to 37 genotypes of sugarcane) in 6 locations throughout the Tucumán sugarcane crop area. The RT-PCR was performed as Yang and Mirkov, (1997), using the primers SCMV R3-F3, and SCMV R3-F4 designed by Alegria et al. (CP) gene by RT-PCR and RFLP. Our objective is to study the genetic diversity of the viruses associated with sugarcane mosaic disease in Tucumán (Argentina), analyzing the sequence variability within the CP-coding region. For this purpose, leaf samples showing severe and common mosaic symptoms, were collected from 61 sugarcane plants (corresponding to 37 genotypes of sugarcane) in 6 locations throughout the Tucumán sugarcane crop area. The RT-PCR was performed as Yang and Mirkov, (1997), using the primers SCMV R3-F3, and SCMV R3-F4 designed by Alegria et al. et al. (2004) analyzing a region of the coat protein (CP) gene by RT-PCR and RFLP. Our objective is to study the genetic diversity of the viruses associated with sugarcane mosaic disease in Tucumán (Argentina), analyzing the sequence variability within the CP-coding region. For this purpose, leaf samples showing severe and common mosaic symptoms, were collected from 61 sugarcane plants (corresponding to 37 genotypes of sugarcane) in 6 locations throughout the Tucumán sugarcane crop area. The RT-PCR was performed as Yang and Mirkov, (1997), using the primers SCMV R3-F3, and SCMV R3-F4 designed by Alegria et al.et al. (2003). Fifty seven samples (93%) resulted positive, 33% of them belong to the strain E of SCMV and the remainder isolates produced nine different RFLP profiles, which did not match with any known strain. The nucleotide sequences of the PCR fragments of these last ones, indicated that 20% belong to the strain D (95% of identity in the CP nucleotide sequence) while the rest showed a great genetic diversity. Besides, using SrMV-specific primers (Yang and Mirkov, 1997) the presence of this virus was detected in 55 leaf samples, finding the existence of both virus in 52 of them. Neither SCMVnor SrMV- were detected in approximately 2% of the samples showing mosaic symptoms, suggesting that other virus may cause sugarcane mosaic symptoms in Tucumán.