Interspecific Crosses Between Wild Species of the Genus Fragaria.

LUQUE, A.C.; DIAZ RICCI, J.C.; CASTAGNARO, A.P.; ARIAS, M.E.

BIOCELL

INST HISTOL EMBRIOL-CONICET

Lugar: Mendoza; Año: 2010 vol. 34 p. 121 - 121

0327-9545

In the Active Germplasm Bank for Strawberry (AGB-UNT) crosseswere made between wild species, relatives of the cultivated strawberry.Successful fertilization events in interspecific crosses aredifficult because of pre-zygotic and post-zygotic barriers to thegenus Fragaria species. For compatibility relations between localFragaria species and their reproductive characteristics, the pollen-pistil interaction and the viability of achenes from Fragariavesca (2n=14) and Fragaria chiloensis (2n=56) are discussed. Interspecificcrosses between F. vesca x F. chiloensis were made inboth directions. Post-pollination pistils were harvested, fixed inFAA, clarified, stained with aniline blue and observed under a UVlight microscope. The achenes were sown in sterile soil. Pistilsanalysis of F. vesca showed pollen germination and pollen tubepenetration at different levels into the style. This cross yieldedconocarpos with achenes which showed a single seedling that diedshortly after. In the reciprocal cross, there was no pollen tube developmentor achenes formation. The achenes obtained by selfingof F. vesca germinated into plants. Prezygotic studies revealedpollen-pistil compatibility in F. vesca x F. chiloensis, but not inreciprocal crosses. However, the non germinated achenes suggestedpost-zygotic barriers. These results provide new information aboutthe reproductive behavior of local genotypes of Fragaria