INVESTIGADORES
CASTAGNARO Atilio Pedro
artículos
Título:
Analysis of the molecular basis of Xanthomonas axonopodis pv. citri
Autor/es:
SICILIANO F, TORRES P, SENDíN L, BERMEJO C, FILIPPONE P, VELLICE G, RAMALLO J, CASTAGNARO A, VOJNOV A AND MARANO MR
Revista:
ELECTRONIC JOURNAL OF BIOTECHNOLOGY
Referencias:
Año: 2006 vol. 9 p. 199 - 204
ISSN:
0717-3458
Resumen:
Xanthomonas axonopodis pathovar citri (Xac) causes
bacterial citrus canker, a serious disease of most citrus
species. Xanthomonas campestris pv. campestris (Xcc) is
the causal agent of black rot disease in cruciferous
plants. In Xcc, cell-cell signaling is mediated by
diffusible signal factor (DSF). Synthesis of DSF depends
on RpfB and RpfF. DSF perception and signal
transduction have been suggested to involve a twocomponent
system comprising RpfC and RpfG. It has
been proposed that these proteins participate in a signal
transduction system linking changes in the environment
to the synthesis of DSF and the expression of virulence
genes. Although the cluster of the rpf genes in Xac has
synteny with the corresponding cluster in Xcc, two
genes (rpfH and rpfI) are absent in Xac. To investigate
DSF-mediated regulation during Xac-Citrus limonpathovar citri (Xac) causes
bacterial citrus canker, a serious disease of most citrus
species. Xanthomonas campestris pv. campestris (Xcc) is
the causal agent of black rot disease in cruciferous
plants. In Xcc, cell-cell signaling is mediated by
diffusible signal factor (DSF). Synthesis of DSF depends
on RpfB and RpfF. DSF perception and signal
transduction have been suggested to involve a twocomponent
system comprising RpfC and RpfG. It has
been proposed that these proteins participate in a signal
transduction system linking changes in the environment
to the synthesis of DSF and the expression of virulence
genes. Although the cluster of the rpf genes in Xac has
synteny with the corresponding cluster in Xcc, two
genes (rpfH and rpfI) are absent in Xac. To investigate
DSF-mediated regulation during Xac-Citrus limonXanthomonas campestris pv. campestris (Xcc) is
the causal agent of black rot disease in cruciferous
plants. In Xcc, cell-cell signaling is mediated by
diffusible signal factor (DSF). Synthesis of DSF depends
on RpfB and RpfF. DSF perception and signal
transduction have been suggested to involve a twocomponent
system comprising RpfC and RpfG. It has
been proposed that these proteins participate in a signal
transduction system linking changes in the environment
to the synthesis of DSF and the expression of virulence
genes. Although the cluster of the rpf genes in Xac has
synteny with the corresponding cluster in Xcc, two
genes (rpfH and rpfI) are absent in Xac. To investigate
DSF-mediated regulation during Xac-Citrus limonXcc, cell-cell signaling is mediated by
diffusible signal factor (DSF). Synthesis of DSF depends
on RpfB and RpfF. DSF perception and signal
transduction have been suggested to involve a twocomponent
system comprising RpfC and RpfG. It has
been proposed that these proteins participate in a signal
transduction system linking changes in the environment
to the synthesis of DSF and the expression of virulence
genes. Although the cluster of the rpf genes in Xac has
synteny with the corresponding cluster in Xcc, two
genes (rpfH and rpfI) are absent in Xac. To investigate
DSF-mediated regulation during Xac-Citrus limonrpf genes in Xac has
synteny with the corresponding cluster in Xcc, two
genes (rpfH and rpfI) are absent in Xac. To investigate
DSF-mediated regulation during Xac-Citrus limonXcc, two
genes (rpfH and rpfI) are absent in Xac. To investigate
DSF-mediated regulation during Xac-Citrus limonrpfH and rpfI) are absent in Xac. To investigate
DSF-mediated regulation during Xac-Citrus limonXac-Citrus limon
interaction, we constructed two strains of Xac, one with
a mutation in the rpfF gene, leading to an inability to
produce DSF, and one with a mutation in the rpfC gene
leading to an overproduction of DSF. These mutants
also show decreased levels of extracellular cyclic â -
(1,2)-glucans and decreased production of
endoglucanase and protease extracellular enzymes. TheXac, one with
a mutation in the rpfF gene, leading to an inability to
produce DSF, and one with a mutation in the rpfC gene
leading to an overproduction of DSF. These mutants
also show decreased levels of extracellular cyclic â -
(1,2)-glucans and decreased production of
endoglucanase and protease extracellular enzymes. TherpfF gene, leading to an inability to
produce DSF, and one with a mutation in the rpfC gene
leading to an overproduction of DSF. These mutants
also show decreased levels of extracellular cyclic â -
(1,2)-glucans and decreased production of
endoglucanase and protease extracellular enzymes. TherpfC gene
leading to an overproduction of DSF. These mutants
also show decreased levels of extracellular cyclic â -
(1,2)-glucans and decreased production of
endoglucanase and protease extracellular enzymes. Theâ -
(1,2)-glucans and decreased production of
endoglucanase and protease extracellular enzymes. The
Xac DSF-deficient rpfF and the DSF-hyper producingDSF-deficient rpfF and the DSF-hyper producing
rpfC mutants are both severely compromised in their
ability to cause canker symptoms in lemon leaves
compared to the wild-type. Here we provide evidence
that rpf genes in Xac are involved in controlling
virulence factors mediated by DSF.mutants are both severely compromised in their
ability to cause canker symptoms in lemon leaves
compared to the wild-type. Here we provide evidence
that rpf genes in Xac are involved in controlling
virulence factors mediated by DSF.rpf genes in Xac are involved in controlling
virulence factors mediated by DSF.