Response surface modeling looking for maximal lipase activity from Ricinus communis powder in hydrolysis reactions

PALLA, CAMILA; SALABERRIA, FLORENCIA; MARÍA E. CARRÍN

Ghent

Congreso; 14th Euro Fed Lipid Congress; 2016

The aim of this work was to evaluate lipase activity in hydrolysis reactions using acetone powders from dormant castor bean seeds as biocatalyst. Reaction media contained 5 mL of acetate buffer (pH 4.4) and 5 mL of an emulsion of arabic gum:water solution (surfactant) and high oleic sunflower oil (substrate). The emulsion composition was determined by the chosen experimental design.A Box-Behnken experimental design was used with a total of 36 runs performed in a random order with 6 replicates at the center point. The levels of each independent variable were chosen based on prior experiments and bibliographic references. Analyzed parameters and their corresponding ranges were: A, amount of lipase powder (0.05-0.15 g); B, arabic gum:water solution ratio (0-6% p/p); and C, oil (0.16-0.5 g Oil/g arabic gum:water solution). Other reaction parameters were fixed (37 ºC, 2 h). The experimental response R was lipase activity (mmol FFA / g Oil ? g Lipase ? h) where FFA stands for free fatty acids. FFA were determined by GC after a solvent extraction from the reaction system. Results were analyzed using Design-Expert 7.0.0 software.The obtained model was significant (p