INVESTIGADORES
CARPINELLA Maria Cecilia
artículos
Título:
Antifungal effects of different organic extracts from Melia azedarach L. on phytopathogenic fungi and their isolated active components
Autor/es:
MARÍA C. CARPINELLA *; LAURA M. GIORDA,; CARLOS G. FERRAYOLI; SARA M. PALACIOS
Revista:
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Editorial:
American Chemical Society
Referencias:
Lugar: Washington DC; Año: 2003 vol. 51 p. 2506 - 2511
ISSN:
0021-8561
Resumen:
Extracts from different parts of Melia azedarach L. were studied as potential antifungal agents for selected phytopathogenic fungi. In a serial agar dilution method, hexanic and ethanolic extracts from fruit, seed kernels and senescent leaves exhibited fungistatic activity against Aspergillus flavus, Diaporthe phaseolorum var. meridionales, Fusarium oxysporum, F. solani, F. verticillioides and Sclerotinia sclerotiorum. Both hexanic extract from senescent leaves and ethanolic extract from seed kernel were highly effective on all tested fungi, with MIC values ranging from 0.5 to 25 mg/mL and 0.5 to 5 mg/mL, respectively. In addition, all the above-mentioned extracts showed fungicidal activity on these fungi, with ethanolic seed kernel extract being the most active. Three compounds displaying activity against F. verticillioides were isolated from the ethanolic seed kernel extract and were characterized as vanillin (1), 4-hydroxy-3-methoxycinnamaldehyde (2) and (±) pinoresinol (3), with MICs of 0.6, 0.4 and 1.0 mg/mL, respectively. These compounds also showed a synergistic effect when combined in different concentrations, needing four times less concentration to reach complete inhibition in the growth of F. verticillioides.