Egg protein ovotransferrin derived peptide IRW on human endothelial inflammatory response during hipoxia

A. M. BIROCCO; A. BLACHMAN; S. L. SAAVEDRA; S. A. CAMPERI; G. C. CALABRESE

Congreso; Reunión Anual de Sociedades de Biociencias 2019.; 2019

Due to the unavoidable side effects of synthetic drugs, there is an increasing interest in the search for novel bioactive food components for the prevention and treatment of cardiovascular diseases. IRW, from egg white protein ovotransferrin, was found to exhibit an anti-inflammatory effect blocking the nuclear translocation of the transcriptional factor NFkB. The objective of the present study was to to examine the molecular mechanisms of its anti-inflammatory activities during hypoxia. IRW is produced by solid phase peptide synthesis. Human endothelial cells line EA.hy926 was incubated in the presence of 5 or 50 µM IRW during 20 h, before adding cobalt chloride (250 µM) for 6 h to induce chemical hypoxia. Endothelial sterile inflammatory response was evaluated by NFκB subcellular distribution through immunofluorescence analysis and zymography studies. Endothelial induced nitric oxide synthase (iNOS) expression was determined by RT-PCR. After hypoxia, NFκB was localized both at the cytoplasmic and nuclear compartment. Nevertheless, the transcriptional factor is restricted to the cytoplasm in the presence of IRW (5 or 50 µM). Simultaneously, the increased activity of metalloproteinase-9 during hypoxia was diminished near of control levels in the presence of IRW . Moreover, hypoxia induced a significative increase in iNOS expression, which was only decreased near to control levels after IRW 5 µM treatment . Our results suggest the IRW potential as functional food ingredient or nutraceutical for the prevention of both endothelial dysfunction and endothelial inflammatory response, key factors for the development of cardiovascular diseases.