High-speed pectic enzyme fractionation by immobilized metal ion affinity membranes

S. A. CAMPERI; M. GRASSELLI; O. CASCONE

Focus on Biotechnology

Kluwer Academic Publishers

Lugar: Holanda; Año: 2000; p. 339 - 349

Immobilised metal ion affinity polysulphone hollow-fibre membranes with a high capacity for protein adsorption were prepared and their application for commercial pectin enzyme fractionation was studied. The pass-through fraction containing pectinlyase (PL) is useful for fruit-juice clarification without methanol production on account of pectin-esterase (PE) being retained by the IDA-Cu2+ membrane.