INVESTIGADORES
CAMPERI Silvia Andrea
artículos
Título:
Screening of one-bead-one-peptide combinatorial library using red fluorescent dyes. Presence of positive and false positive beads
Autor/es:
M. M. MARANI; M. C. MARTÍNEZ-CERON; S. L. GIUDICESSI; E. DE OLIVEIRA; S. CÔTÉ; R. ERRA-BALSELLS; F. ALBERICIO; O. CASCONE; S. A. CAMPERI
Revista:
J. Comb. Chem.
Editorial:
AMER CHEMICAL SOC
Referencias:
Lugar: Washington; Año: 2009 vol. 11 p. 146 - 146
ISSN:
1520-4766
Resumen:
To screen one-bead-one-compound (OBOC) combinatorial libraries, tens of thousands to millions of compound beads are first mixed with a target molecule. The beads that interact with the target molecule will be identified and then isolated for compound structure determination. Herein we describe an OBOC peptide library screening using streptavidin (SA) as probe protein, labeled with a red fluorescent dye and using the COPASTM BIO-BEAD flow sorting equipment to separate fluorescent from non-fluorescent beads. Red dyes investigated were ATTO 590 and Texas Red. After incubating the library with the SA-red fluorescent dye conjugate, positive beads due to peptide-SA interaction and false positive beads due to peptide-fluorescent dye interaction were isolated. Those false positives were a drawback when sorting beads with COPAS. However, a deep analysis of both allows differentiating positive from false positive.  The false positive had a bright homogeneous fluorescence while positive beads had a heterogeneous fluorescence exhibiting a characteristic halo appearance, with fluorescence intensity greatest at the bead surface and lowest in the core. The difference was more evident when using Texas Red instead of ATTO 590. Thus, positive from false positive beads could be manually isolated. The beads were analyzed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Most of the sequences obtained from positive beads had the His-Pro-Gln motif. Peptides from false positive beads were rich in Leu/Ileu, His, Phe and Tyr.
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