Affinity Chromatography based on a Combinatorial Strategy for rErythropoietin Purification

M. C. MARTÍNEZ-CERON; M. M. MARANI; M. TAULÉS; M. ETCHEVERRIGARAY; F. ALBERICIO; O. CASCONE; S. A. CAMPERI

ACS Combinatorial Science

American Chemical Society

Año: 2011 vol. 13 p. 251 - 258

2156-8952

Small peptides containing fewer than ten amino acids are promising ligand candidates to build affinity chromatographic systems for industrial protein purification. The application of combinatorial peptide synthesis strategies greatly facilitates the discovery of suitable ligands for any given protein of interest. Here we sought to identify peptide ligands with affinity for recombinant human erythropoietin (rhEPO), which is used for the treatment of anemia. A combinatorial library containing the octapeptides X-X-X-Phe-X-X-Ala-Gly, where X=