Application of Z-sinapinic matrix in peptide MALDI-MS analysis

SALUM, M.L.; GIUDICESSI, S.L.; SCHMIDT DE LEÓN, T.; CAMPERI, S.A.; ERRA-BALSELLS, R.

JOURNAL OF MASS SPECTROMETRY

JOHN WILEY & SONS LTD

Año: 2017 vol. 52 p. 182 - 186

1076-5174

Since introduction of sinapinic acid (SA) and α-cyano-4-hydroxycinnamic acid as matrices, successful application of matrix-assisted laser desorption/ionization mass spectrometry started for protein/polypeptides. Both show some limitations in short peptide analysis because matrix clusters are quite abundant. Cinnamics currently used are E-cinnamics. Here, Z-SA as matrix for peptides is studied and compared with E-SA and α-cyano-4-hydroxycinnamic acid. Minor number of clusters is always observed in the low m/z region allowing the detection of short peptides. The results here described show that this novel matrix is a tool of choice for direct, rapid and sensitive detection of hydrophilic and hydrophobic peptides. Copyright © 2017 John Wiley & Sons, Ltd.