INVESTIGADORES
CAMPERI Silvia Andrea
artículos
Título:
Fungal pectic enzyme fractionation by dye affinity chromatography
Autor/es:
SILVIA A. CAMPERI; MARIANO GRASSELLI; OSVALDO CASCONE
Revista:
BIOTECHNOLOGY TECHNIQUES
Editorial:
Kluwer Academic Publishers
Referencias:
Lugar: The Netherlands; Año: 1996 vol. 10 p. 211 - 211
ISSN:
0951-208X
Resumen:
A dye affinity fractionation of a commercial pectic enzyme preparation is described. From nine immobilized triazinic dyes assayed, only Blue R-HE and Yellow 4R-HE were able to retain pectin lyase thus allowing the obtention of a fraction not producing methanol during fruit juice clarification. While pectinesterase did not bind to the chromatographic matrix, the retained pectin lyase could be quantitatively eluted with a salt gradient.
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