CAMPERI Silvia Andrea
Fungal pectic enzyme fractionation by dye affinity chromatography
SILVIA A. CAMPERI; MARIANO GRASSELLI; OSVALDO CASCONE
Kluwer Academic Publishers
Lugar: The Netherlands; Año: 1996 vol. 10 p. 211 - 211
A dye affinity fractionation of a commercial pectic enzyme preparation is described. From nine immobilized triazinic dyes assayed, only Blue R-HE and Yellow 4R-HE were able to retain pectin lyase thus allowing the obtention of a fraction not producing methanol during fruit juice clarification. While pectinesterase did not bind to the chromatographic matrix, the retained pectin lyase could be quantitatively eluted with a salt gradient.