Unmasking the scent of Hypsiboas pulchellus (Anura: Hylidae) using in vivo headspace solid phase microextraction

ANDRÉS E. BRUNETTI; GABRIELA M. CABRERA; JULIÁN FAIVOVICH; JOSIAS MERIB; EDUARDO CARASEK; JANAINA BARBARÁ; PATRICK COLOMBO; CLAUDIA ZINI

Simposio; 15th International Symposium on Advances in Extraction Technologies; 2013

At the light of evidences, the aim of present work is to identify the volatiles molecules secreted by H. pulchellus by using SPME, and try to establish a relationship among them and the type of odor that is perceived by humans. Nine low molecular weight compounds were tentatively identified: two alkenes, one aldehyde, two ketones, one alcohol, one ester, and two sulfides (Fig. 2). Different extraction conditions were needed to catch some of the compounds present in the frogs´ secretions (different extraction times, and live frogs vs. skins alone). These tentatively identify molecules could account for the herbaceous, pungent, and skunk like odor perceived by different people.