INVESTIGADORES
CABRERA Gabriela Myriam
artículos
Título:
Cyclosporine A from a nonpathogenic Fusarium oxysporum suppressing Sclerotinia sclerotiorum
Autor/es:
RODRIGUEZ, MARÍA ALEJANDRA; GABRIELA MYRIAM CABRERA; GODEAS, ALICIA
Revista:
JOURNAL OF APPLIED MICROBIOLOGY
Editorial:
Soc. Appl. Microbiol.
Referencias:
Año: 2006 vol. 100 p. 575 - 586
ISSN:
1364-5072
Resumen:
Aims: To evaluate the antagonistic activity of Fusarium oxysporum nonpathogenic
fungal strain S6 against the phytopathogenic fungus Sclerotinia sclerotiorum
fungal strain S6 against the phytopathogenic fungus Sclerotinia sclerotiorum
and to identify the antifungal compounds involved.
Methods and Results: The antagonistic activity of Fusarium oxysporum strain
S6 was determined in vitro by dual cultures. The metabolite responsible for the
activity was isolated by chromatographic techniques, purified and identified by
spectroscopic methods as cyclosporine A. The antifungal activity against the
pathogen was correlated with the presence of this metabolite by a dilution
assay and then quantified. Cyclosporine A caused both growth inhibition and
suppression of sclerotia formation. In a greenhouse assay, a significant increase
in the number of surviving soybean (Glycine max) plants was observed when
activity was isolated by chromatographic techniques, purified and identified by
spectroscopic methods as cyclosporine A. The antifungal activity against the
pathogen was correlated with the presence of this metabolite by a dilution
assay and then quantified. Cyclosporine A caused both growth inhibition and
suppression of sclerotia formation. In a greenhouse assay, a significant increase
in the number of surviving soybean (Glycine max) plants was observed when
activity was isolated by chromatographic techniques, purified and identified by
spectroscopic methods as cyclosporine A.
S6 was determined in vitro by dual cultures. The metabolite responsible for the
activity was isolated by chromatographic techniques, purified and identified by
spectroscopic methods as cyclosporine A. The antifungal activity against the
pathogen was correlated with the presence of this metabolite by a dilution
assay and then quantified. Cyclosporine A caused both growth inhibition and
suppression of sclerotia formation. In a greenhouse assay, a significant increase
in the number of surviving soybean (Glycine max) plants was observed when
activity was isolated by chromatographic techniques, purified and identified by
spectroscopic methods as cyclosporine A. The antifungal activity against the
pathogen was correlated with the presence of this metabolite by a dilution
assay and then quantified. Cyclosporine A caused both growth inhibition and
suppression of sclerotia formation. In a greenhouse assay, a significant increase
in the number of surviving soybean (Glycine max) plants was observed when
activity was isolated by chromatographic techniques, purified and identified by
spectroscopic methods as cyclosporine A.