Multiple response optimization of a QuEChERS extraction and HPLC analysis of diclazuril, nicarbazin and lasalocid in chicken liver

SILVA, JUAN MANUEL; AZCÁRATE, FEDERICO JOSÉ; KNOBEL, GASTON; SOSA, JESICA SOLEDAD; CARRIZO, DAIANA BELÉN; BOSCHETTI, CARLOS EUGENIO

FOOD CHEMISTRY

ELSEVIER SCI LTD

Año: 2020 vol. 311 p. 126014 - 126020

0308-8146

A method for the simultaneous determination of three commonly used coccidiostats in chicken liver was developed,comprising a multi-residue QuEChERS (quick, easy, cheap, effective, rugged and safe) extraction step,and a liquid chromatography-ultra violet-fluorescence (HPLC-UV/FL) analysis. The QuEChERS extraction wasoptimized using an experimental design approach that includes a screening step to obtain the critical variables,an optimization step using multiple response surface analysis and the calculation of a desirability parameter. Theoptimized method was validated with fortified samples, reaching an average recovery of 91% and an overallprecision of 5.5% (mean of three analytes at three levels). Limits of detection calculated on fortified sampleswere 20 μg kg−1 for lasalocid, 15 μg kg−1 for nicarbazin and 120 μg kg−1 for diclazuril. These values resulted atleast one order of magnitude lower than the maximum allowed residue limit (MRL) of the studied coccidiostatsfor chicken liver