INVESTIGADORES
BOLONTRADE Marcela Fabiana
congresos y reuniones científicas
Título:
Expression of Granulocyte Colony-Stimulating Factor and its receptor in Ewing’s Sarcoma
Autor/es:
MORALES-ARIAS, JAIME, KOSHKINA, NADEZHDA V., ZHOU, ZHICHAO; BOLONTRADE, MARCELA F.; KLEINERMAN, EUGENIE S.
Lugar:
Anaheim, California
Reunión:
Simposio; 96 vo Encuentro Anual - American Association for Cancer Research (AACR).; 2005
Institución organizadora:
American Association for Cancer Research (AACR).
Resumen:
Ewing’s sarcoma (ES) is a highly vascular malignancy.  We have demonstrated that both angiogenesis and vasculogenesis contribute to the growth of Ewing’s tumors.  Granulocyte colony-stimulating factor (G-CSF), a cytokine known to stimulate bone marrow (BM) stem cell production and angiogenesis, is routinely administered to ES patients after chemotherapy. We therefore examined whether ES cells express G-CSF and its receptor and whether treatment with this factor enhances tumor growth. Human ES cell lines were analyzed for expression of G-CSF and G-CSFR in vitro and in vivo.  Sixty-eight paraffin-embedded and 15 frozen tumor specimens from ES patients were also evaluated for the presence of G-CSF and G-CSFR.  The in vivo effect of G-CSF on angiogenesis and BM cell migration was determined.  Using a TC/7-1 human ES mouse model, the effect of G-CSF administration on ES tumors was studied. Expression of both G-CSF and G-CSFR protein and RNA was identified in all ES cell lines and patient samples analyzed.  Additionally, G-CSF was found to stimulate angiogenesis and BM cell migration in vivo.  In G-CSF-treated mice, tumor growth was significantly increased.  The average tumor volume for the G-CSF-treated group was 1,218 mm3 compared to 577 mm3 for the control group (P =0.006). Since ES cells and patient tumor specimens expressed both G-CSF and its receptor in vitro and in vivo and administration of G-CSF promoted tumor growth in vivo, our data suggest that caution is warranted when administering G-CSF to patients with ES.