INVESTIGADORES
BOLONTRADE Marcela Fabiana
congresos y reuniones científicas
Título:
Role of Bone Marrow Derived Cells in the Development of Ewing’s Sarcoma.
Autor/es:
BOLONTRADE, MARCELA F.; ZHOU, RONG R.; ANDREEFF, MICHAEL; WEIDNER, DOUGLAS A.; KLEINERMAN, EUGENIE S.
Lugar:
New Orleans, Louisiana.
Reunión:
Simposio; 92do Encuentro Anual. American Association for Cancer Research (AACR).; 2001
Institución organizadora:
American Association for Cancer Research (AACR).
Resumen:
Formation of new vessels that support tumor growth may involve vasculogenesis, as well as angiogenesis. Vasculogenesis, in contrast to angiogenesis, is the development of a vascular network from endothelial cell precursors. We investigated the role of bone marrow (BM) cells in the development of vessels and stroma of Ewing´s sarcomas. Human TC71 Ewing´s sarcoma cells form tumors 2 ½ weeks following injection into the bone. BM cells from mice with Ewing´s tumors and control mice were analyzed by FACS using UV excitation and dual wavelength emission of cells stained with the dye Hoechst 33342. Side population (SP) cells were identified using this technique, which have been characterized as stem cells (Goodell et al, J exp Med 1996; 183(4): 1797-906). The percentage of SP cells in the BM was significantly higher in tumor bearing mice than in controls (0.396 +- 0.246% vs. 0.045 +- 0.035%, p= 0.05). We further demonstrated that unfractioned BM cells stained with CM-DiI were incorporated into the vasculature and surrounding stroma of developing Ewing´s tumors in vivo following i.v. injection. These data indicate that bone marrow cells are present in the tumor vasculature and stroma, which may contribute to the growth of Ewing´s sarcoma in mice. Furthermore, Ewing´s tumor cells may induce the bone marrow to produce cells to support the vasculogenesis needed by the growing tumor.