Incorporation of Mesenchymal Stem Cells into Areas of Lung Metastasis in an Osteosarcoma Model.

GUTIÉRREZ, LUCIANA; AMORÓS, MARIANA A.; SEVLEVER, GUSTAVO; PODHAJCER, OSVALDO ; CREMASCHI, GRACIELA; KLEINERMAN, EUGENIE S. ; BOLONTRADE, MARCELA F.

Philadelphia

Simposio; 106th Annual Meeting- American Association for Cancer Research (AACR); 2015

American Association for Cancer Research

Lung is the most common metastatic site in osteosarcoma patients with a majority presenting lung micrometastasis at diagnosis time. MSCs compose a heterogeneous cell population with the ability to migrate and home into remodeling tissues, including tumors. To evaluate the participation ofMSCs in lung metastasis formation, we intravenously injected LM7 osteosarcoma cells into immunosuppressed mice. Pre-labelled MSCs (DiR+/CMDiI+, at a dose of 0.5-1x10e6 cells) were intravenously administered 10 weeks after tumor cells injection, when microscopic metastasis become apparent. MSCs biodistribution was evaluated both through real time (DiR+) and microscopic (CMDiI+) analysis. MSCs levels in the lungs of tumor-bearing mice as compared to mice with no tumor burden was 1.15x10E5±0.07 p2/sec/cm2/sr vs. 5.44x10E4±0.5p2/sec/cm2/sr(one week post-inoculation, infrared signal, p